26.11 Red cell folate

Red cell folate data available for STOP cohort only.

26.11.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	43.64894	1	<0.0001	***
ns(rcfolate, 3)	5.76169	3	0.1	ns
GA_samp	70.48397	1	<0.0001	***

hPL (μg/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	10.1918551	1	0.001	**
ns(rcfolate, 3)	0.1840576	3	1.0	ns
GA_samp	1109.0784596	1	<0.0001	***

GH2 (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	94.062933	1	<0.0001	***
ns(rcfolate, 3)	6.776213	3	0.08	ns
GA_samp	558.762160	1	<0.0001	***

Insulin (uU/ml)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate	1	Inf	0.363	0.363	0.5	ns
GA_samp	1	Inf	0.389	0.389	0.5	ns

HOMA-IR

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate	1	Inf	0.345	0.345	0.6	ns
GA_samp	1	Inf	0.266	0.266	0.6	ns

Glucose (mmol/L)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate	1	Inf	0.171	0.171	0.7	ns
GA_samp	1	Inf	1.236	1.236	0.3	ns

26.11.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)

Var	Effect	P
(Intercept)	17.37 (7.46, 40.43)	<0.0001
ns(rcfolate, 3)1	0.80 (0.49, 1.32)	0.39
ns(rcfolate, 3)2	0.89 (0.18, 4.41)	0.89
ns(rcfolate, 3)3	1.01 (0.80, 1.27)	0.92
GA_samp	1.15 (1.11, 1.18)	<0.0001

hPL (μg/ml)

Var	Effect	P
(Intercept)	2.13 (1.34, 3.40)	0.001
ns(rcfolate, 3)1	0.95 (0.72, 1.25)	0.72
ns(rcfolate, 3)2	0.87 (0.36, 2.10)	0.75
ns(rcfolate, 3)3	0.99 (0.87, 1.12)	0.86
GA_samp	1.35 (1.33, 1.37)	<0.0001

GH2 (ng/ml)

Var	Effect	P
(Intercept)	0.04 (0.02, 0.08)	<0.0001
ns(rcfolate, 3)1	0.93 (0.64, 1.37)	0.73
ns(rcfolate, 3)2	1.52 (0.44, 5.27)	0.51
ns(rcfolate, 3)3	0.90 (0.75, 1.07)	0.24
GA_samp	1.35 (1.32, 1.38)	<0.0001

Insulin (uU/ml)

Var	Effect	P
(Intercept)	11.14 (4.71, 26.36)	<0.0001
ns(rcfolate, 3)1	0.80 (0.48, 1.31)	0.37
ns(rcfolate, 3)2	0.65 (0.13, 3.15)	0.59
ns(rcfolate, 3)3	1.07 (0.85, 1.35)	0.57
GA_samp	1.01 (0.97, 1.06)	0.53

HOMA-IR

Var	Effect	P
(Intercept)	2.42 (0.93, 6.32)	0.07
ns(rcfolate, 3)1	0.79 (0.45, 1.38)	0.41
ns(rcfolate, 3)2	0.63 (0.10, 3.76)	0.61
ns(rcfolate, 3)3	1.09 (0.85, 1.40)	0.50
GA_samp	1.01 (0.97, 1.06)	0.61

Glucose (mmol/L)

Var	Effect	P
(Intercept)	5.04 (4.04, 6.30)	<0.0001
ns(rcfolate, 3)1	1.00 (0.88, 1.13)	1.00
ns(rcfolate, 3)2	0.96 (0.60, 1.52)	0.86
ns(rcfolate, 3)3	1.06 (1.00, 1.13)	0.05
GA_samp	1.00 (0.99, 1.00)	0.27

26.11.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

26.11.4 Plots